10678   Rhox2a     Status: ES cell colonies screened / QC no positives

GeneRhox2a
ChromosomeX
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1922449
NCBI Gene ID75199
BAC ID228G3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF37,245,57537,245,596GGTTGGCAACAGAGCTTCAATG
TUP37,245,59737,245,622TGCTGCAACTACAAGAGCTGGAGAGC
TUR37,245,64037,245,661CGCCTCCTTAGTGCTGATGTAG
TDF37,248,95037,248,974CCAAGAAATCACTGTTTCCTTGTGA
TDP37,249,00137,249,027TTCCCAAATGATCTCTGAGCCTTGGGA
TDR37,249,02837,249,048CATACACACCACGTGTCCTCC

PCR Primers

PrimerSequence
SUAGAGGAACCATCATTTCCTTC
SDTCCTTGAAACAGATCTTCTCG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR87 bp++-
TDFTDR99 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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