10683 Nek8 Status: Cancelled
| Gene | Nek8 |
| Chromosome | 11 |
| Deletion Start | 78,176,603 |
| Deletion End | 78,166,726 |
| Deletion Size | 9,878 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,176,481 | 78,176,501 | CAAGAAATCGTGGCCCTGTTG |
| TUP | 78,176,450 | 78,176,473 | CCCGAAGCCAGAGCACCACTATGG |
| TUR | 78,176,406 | 78,176,425 | GCCTCCCGCCTAAACCTAAG |
| TDF | 78,166,907 | 78,166,928 | ACTTCCTGGAGCCAGTTGATAG |
| TDP | 78,166,879 | 78,166,903 | AACTTCCCTTTCTGGCCTGGCAGGA |
| TDR | 78,166,831 | 78,166,850 | GCAAGCATCAGCCCTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCCAGTCTAACTACCTATGCC |
| SD | AGCAAAGGTTTTGAAGGCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 410 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
