10685 Krtap3-3 Status: ES cell colonies screened / QC no positives
| Gene | Krtap3-3 |
| Chromosome | 11 |
| Deletion Start | 99,550,806 |
| Deletion End | 99,550,480 |
| Deletion Size | 327 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,566,510 | 99,566,531 | CCATCTGCTCCTCAGACAAGTC |
| TUP | 99,566,489 | 99,566,509 | CTGCCGCTGTGGAGTCTGCCT |
| TUR | 99,556,674 | 99,556,692 | GCTGCCAAATGGTGTGTGG |
| TDF | 99,556,555 | 99,556,575 | GACCTGCTGACTGTCAACCTC |
| TDP | 99,556,531 | 99,556,553 | CCACCTATGTGCAGCCAGGCTGT |
| TDR | 99,556,511 | 99,556,530 | CTTGGGACACAGGGCTCCTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCAAGTGTAATCAGTGAAG |
| SD | ACACCCAAAGAAAGGCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 9858 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 342 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
