10686 Trim14 Status: Cancelled
| Gene | Trim14 |
| Chromosome | 4 |
| Deletion Start | 46,536,077 |
| Deletion End | 46,506,851 |
| Deletion Size | 29,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,535,640 | 46,535,660 | CAGGAGGCTCAAGCTGGTTAG |
| TUP | 46,535,613 | 46,535,638 | ACTCACACGTTCTTTACCGCTTCTGC |
| TUR | 46,535,580 | 46,535,599 | CGCGTCCTTGACTCTGGTTG |
| TDF | 46,507,030 | 46,507,048 | CGGCGTCTTCCTGGACTAC |
| TDP | 46,507,002 | 46,507,025 | CCGGCATTCTGGCCTTCTACGACG |
| TDR | 46,506,965 | 46,506,982 | GCGTGGAAGGTGTGCAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGAGCCCATCAGTGCAAG |
| SD | GGACAAGCTGAAGTGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
