| Gene | Olfr159 |
| Chromosome | 4 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,770,549 | 43,770,569 | CGGCTCCTGGGTATCTGGAAG |
| TUP | 43,770,498 | 43,770,527 | ATCTTTAGCAATGACACTGCCCTTCTGTGG |
| TUR | 43,770,433 | 43,770,452 | TGTCAGCACAGGCCAGTTTC |
| TDF | 43,770,080 | 43,770,098 | GAAGGCCGCTGTGACAAAC |
| TDP | 43,770,053 | 43,770,079 | CTGGTGGGCCAGAAACACTTCAAGTGG |
| TDR | 43,770,015 | 43,770,037 | ACAGGGAGACAAAGACCATGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCAAGGGTTAGATACCAC |
| SD | CTGAGACCCTGTTCCCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
