10689 Mrgprb2 Status: ES cell colonies screened / QC no positives
| Gene | Mrgprb2 |
| Chromosome | 7 |
| Deletion Start | 48,552,972 |
| Deletion End | 48,551,828 |
| Deletion Size | 1,145 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,552,810 | 48,552,832 | CCTGGTTGGGATGGGACTAAATG |
| TUP | 48,552,786 | 48,552,809 | CCATAGTGCTGTGGTTCCTGGGCA |
| TUR | 48,552,731 | 48,552,750 | TCAGCCATAGCCAGGTTGAG |
| TDF | 48,552,320 | 48,552,341 | TGCTTGTCAGGATCTTCTGTGG |
| TDP | 48,552,286 | 48,552,315 | CAGCGAATTCCTATGACCAGGCTGTATGTG |
| TDR | 48,552,256 | 48,552,278 | CAGGAAGACCAAGACTGTGAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGCCTGAGACACTGAGTGTC |
| SD | CTTAAACATTGAGAAGGGTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 426 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
