10690 Serpinb9b Status: ES cell colonies screened / QC positives
| Gene | Serpinb9b |
| Chromosome | 13 |
| Deletion Start | 33,121,315 |
| Deletion End | 33,131,942 |
| Deletion Size | 10,628 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,121,355 | 33,121,375 | GGCACCTTTGCCATCCATCTA |
| TUP | 33,121,377 | 33,121,406 | TGAAGATGTTATGTCAAAGCAACCCTTCCA |
| TUR | 33,121,460 | 33,121,479 | GTCTGCTCCTTTGCACCCAG |
| TDF | 33,131,849 | 33,131,869 | GTGAGTTCACACTCCAGCATC |
| TDP | 33,131,871 | 33,131,893 | CCACCACTCCTACAGCTCCGTGA
|
| TDR | 33,131,894 | 33,131,912 | TCCCTCTGTTTGCCCATTC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGTTGCTGAAGGCAGG |
| SD | CTTGTACGGTGAAATCACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 549 bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 46 bp | + | + | - |
| NeoFwd | SD | 252 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
