10691 Cd164 Status: ES cell colonies screened / QC no positives
| Gene | Cd164 |
| Chromosome | 10 |
| Deletion Start | 41,519,538 |
| Deletion End | 41,528,876 |
| Deletion Size | 9,339 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,520,085 | 41,520,105 | CAGCGAGCCTCCTGTAACTTC |
| TUP | 41,520,112 | 41,520,138 | ACTTAGCTGAGCGGTGTTGGCTGTATC |
| TUR | 41,520,149 | 41,520,170 | AGCCTCGTTCTTCCCTTACCAC |
| TDF | 41,528,674 | 41,528,696 | GGGACAACAAATACCACACTGAC |
| TDP | 41,528,697 | 41,528,717 | TCCAACCTCCCAGCCTGAGCG |
| TDR | 41,528,745 | 41,528,766 | GACAAGGACGATCCCTCCAATG |
PCR Primers
| Primer | Sequence |
| SU | CTCCCAGGACAAGAAGGAC |
| SD | GCAGCCATGGTAAACAAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 308 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
