| Gene | Ifna9 |
| Chromosome | 4 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,592,167 | 88,592,186 | ATGCCCAGCAGATCCAGGAG |
| TUP | 88,571,554 | 88,571,577 | TCAAGCCATCCCTGTCCTGAGTGA |
| TUR | 88,592,081 | 88,592,101 | GCATTCCAAGCAGCAGATGAG |
| TDF | 88,591,850 | 88,591,870 | GAGAGCCCTGTCTTCCTCAGT |
| TDP | 88,591,823 | 88,591,848 | AACTTGCTGGCAAGACTGAGTGAGGA |
| TDR | 88,591,661 | 88,591,686 | CTTTACAGATAGACACAGCAAAGCAC |
PCR Primers
| Primer | Sequence |
| SU | GGAGCAGTGAAAGAGGAAGC |
| SD | GATCCCATGAGAGCTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 210 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
