10696 Mgl2 Status: Cancelled
| Gene | Mgl2 |
| Chromosome | 11 |
| Deletion Start | 70,134,158 |
| Deletion End | 70,137,113 |
| Deletion Size | 2,956 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,134,241 | 70,134,264 | GGAGTCTCCAAAGTTTGCTCTAAG |
| TUP | 70,134,267 | 70,134,290 | TGTCTATCAGGGTTTGCGCTGCCG |
| TUR | 70,134,327 | 70,134,350 | CCCTTGAATGTGTAATGAGCCTTG |
| TDF | 70,136,252 | 70,136,276 | TCTGTCTGATTTAACCGACCATGTA |
| TDP | 70,136,280 | 70,136,303 | CAGCTGGAGACAGACTTGAAGGCC |
| TDR | 70,136,336 | 70,136,360 | TCCATCAAACTTCTCATTCTCACCA |
PCR Primers
| Primer | Sequence |
| SU | AGGATGGCCTCGAACTGAG |
| SD | TTACTTGACAATCACAGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 275 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
