10697   Zbtb8b     Status: ES cell colonies screened / QC no positives

GeneZbtb8b
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:2387181
NCBI Gene ID215627
BAC ID418C4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF129,432,474129,432,495TCTGACCTACAACAGCTACCAC
TUP129,432,447129,432,472TGAAGCAGTTCCTGGAGGCTCTCCTG
TUR129,432,393129,432,413GCCACGAGAAGAGTGACAATC
TDF129,427,675129,427,695GACGACCTGATGCCTGTCAAC
TDP129,427,649129,427,672TAGCCTGGTGGAGGCTTCATCTGA
TDR129,427,612129,427,632GATGGGCCAGTCATCTGTGTC

PCR Primers

PrimerSequence
SUCAGGCTAGCCTGGGCTACAG
SDGAGTCAGCTTCTCAGCAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR103 bp++-
TDFTDR84 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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