| Gene | Gp9 |
| Chromosome | 6 |
| Deletion Start | 87,779,008 |
| Deletion End | 87,779,636 |
| Deletion Size | 629 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,779,017 | 87,779,036 | GGCCTCCTGTTTCTGCTCTG |
| TUP | 87,779,040 | 87,779,064 | TGCGACCACAGATACTCAGGCTTGC |
| TUR | 87,779,146 | 87,779,165 | CGAGTGTGCGCTGGTATCAC |
| TDF | 87,779,495 | 87,779,516 | TGCTGGCTGGTCTATTGAACAC |
| TDP | 87,779,521 | 87,779,546 | ACAGAGTCCAGGAACTGAGCCTAGCC |
| TDR | 87,779,589 | 87,779,607 | TGATGGCATGGGCTGACTG |
PCR Primers
| Primer | Sequence |
| SU | CGAAGACTGGAGTCCCTTAG |
| SD | GAGAGTAGCCTCAGTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 360 bp | - | + | + |
| TUF | TUR | 149 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10702A-A10 | VGB6 | C57BL/6NTac | |
| 10702A-B2 | VGB6 | C57BL/6NTac | |
| 10702A-C7 | VGB6 | C57BL/6NTac | |
| 10702A-C12 | VGB6 | C57BL/6NTac | |
| 10702A-D4 | VGB6 | C57BL/6NTac | |
| 10702A-D10 | VGB6 | C57BL/6NTac | |
