10703 Ifna1 Status: Cancelled
| Gene | Ifna1 |
| Chromosome | 4 |
| Deletion Start | 88,850,071 |
| Deletion End | 88,850,656 |
| Deletion Size | 586 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,850,189 | 88,850,213 | AAGAGAGCCTTGACACTCCTGGTAC |
| TUP | 88,850,238 | 88,850,264 | CCTGCCTGAAGGACAGGAAGGACTTTG |
| TUR | 88,850,300 | 88,850,320 | AGGGATGGCTTGAGCCTTCTT |
| TDF | 88,850,482 | 88,850,501 | TCCCCTGACCCAGGAAGATG |
| TDP | 88,850,503 | 88,850,529 | CCTGCTGGCTGTGAGGAAATACTTCCA |
| TDR | 88,850,582 | 88,850,602 | CCAGACTTCTGCTCTGACCAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTTTGATGAGGACCAGTG |
| SD | AGAGAACAAGTGCCTTTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | 225 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
