10704 Chad Status: Cancelled
| Gene | Chad |
| Chromosome | 11 |
| Deletion Start | 94,565,082 |
| Deletion End | 94,568,533 |
| Deletion Size | 3,452 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,565,742 | 94,565,763 | CAAACTTCGGGTGGTGGAGGAG |
| TUP | 94,565,765 | 94,565,791 | TGAAGCTGTCTCACAACCCTCTGAAGA |
| TUR | 94,565,797 | 94,565,817 | GGACTGGAAGGCATTGTCTGG |
| TDF | 94,567,826 | 94,567,846 | CCACACTGAAACACGTCCATC |
| TDP | 94,567,847 | 94,567,868 | TGGACAACAACCGCCTGAACCA |
| TDR | 94,567,891 | 94,567,910 | GTGAGGGTCTCCAGGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | AACAGGAACCAAGGGGTTGG |
| SD | TCTGTGTCTTTCCTAGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
