10713 Fpr3 Status: Cancelled
| Gene | Fpr3 |
| Chromosome | 17 |
| Deletion Start | 17,970,453 |
| Deletion End | 17,971,543 |
| Deletion Size | 1,091 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,970,826 | 17,970,845 | GCCTTGGACCGCTGTATTTG |
| TUP | 17,970,846 | 17,970,866 | TGTCCTGCATCCAGTCTGGGC |
| TUR | 17,970,896 | 17,970,917 | CAGGACCCAACAACCACATTTC |
| TDF | 17,971,179 | 17,971,201 | CCGTCCTTTCCGAGTTCTTACAG |
| TDP | 17,971,206 | 17,971,235 | TGTGGCTTCCTTCTTTATCTGTTGGTTTCC |
| TDR | 17,971,264 | 17,971,284 | CTGCATCTCTTTGAGCCAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGTCTGAAAATCTTGAATTG |
| SD | CTCTTCCTACAACTAACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
