| Gene | Gzmg |
| Chromosome | 14 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,159,145 | 56,159,168 | GGCAAGGCAGTTTTAATGACCAGT |
| TUP | 56,159,107 | 56,159,133 | TGAGGCCCCCAGCTCAATAATCTTAGC |
| TUR | 56,159,030 | 56,159,047 | GTGGAGCGTGCAGTGCAG |
| TDF | 56,156,779 | 56,156,803 | CAACCTAGCTTATGGAGTTGTATCC |
| TDP | 56,156,741 | 56,156,770 | AAACAGGACAATCACTCCAGGAGTCTTCAC |
| TDR | 56,117,747 | 56,117,767 | GCTTATCCACGGCAGGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CCTCCTCAACCACAACCTCC |
| SD | CCATGTATGGGTGATAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 139 bp | + | + | - |
| TDF | TDR | 39057 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
