10715 Nkx2-4 Status: ES cell colonies screened / QC no positives
| Gene | Nkx2-4 |
| Chromosome | 2 |
| Deletion Start | 147,085,342 |
| Deletion End | 147,083,876 |
| Deletion Size | 1,467 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,085,305 | 147,085,324 | ACGACGCCCTTCTCCGTTTC |
| TUP | 147,085,282 | 147,085,304 | CGACATCCTGAGCCCCATCGAGG |
| TUR | 147,085,261 | 147,085,280 | CGCCGAACTTCTTGTAGGTC |
| TDF | 147,084,260 | 147,084,278 | GGCCAGCATGATCCACCTG |
| TDP | 147,084,236 | 147,084,258 | CGCCCACGCAGGTCAAGATCTGG |
| TDR | 147,084,200 | 147,084,221 | GGCCTGCCTCTTCATCTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCAGAAACACCCAACTG |
| SD | TCTTCCGCATAACTTAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 441 bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
