10718 Sox30 Status: ES cell colonies screened / QC no positives
| Gene | Sox30 |
| Chromosome | 11 |
| Deletion Start | 45,980,285 |
| Deletion End | 45,992,095 |
| Deletion Size | 11,811 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,981,096 | 45,981,114 | CACGTCCAATGGCCTGGTC |
| TUP | 45,981,115 | 45,981,137 | CACAGCAGCAAGGAGGCCATCCT |
| TUR | 45,981,188 | 45,981,208 | GGACGGTGTGGAGTGTCAAAG |
| TDF | 45,991,851 | 45,991,870 | GGAGAGCACCACCAGGATTC |
| TDP | 45,991,872 | 45,991,896 | AGCTGGTGCAAGTACTGCCCATGCC |
| TDR | 45,991,930 | 45,991,949 | GTGGAAACGCTGGCATACTC |
PCR Primers
| Primer | Sequence |
| SU | AAAGAGAATCCGGAAGAGAG |
| SD | GGCTGGAGCGGAGAAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 419 bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
