10721 Txndc8 Status: ES cell colonies screened / QC one positive
| Gene | Txndc8 |
| Chromosome | 4 |
| Deletion Start | 58,009,104 |
| Deletion End | 57,984,168 |
| Deletion Size | 24,937 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,008,578 | 58,008,599 | ACATGAGAAGCCAGGCTTGGTG |
| TUP | 58,008,550 | 58,008,575 | ACAGGCTGGTACTTCCAGCTACTCAA |
| TUR | 58,008,481 | 58,008,503 | GCTGGACCTTGTCTTGTATTCAC |
| TDF | 57,985,142 | 57,985,162 | GCCAGAGAACAACTTCAGCAC |
| TDP | 57,985,113 | 57,985,137 | CCTCGGGAATCATCCGTCTCGATGG |
| TDR | 57,985,045 | 57,985,065 | TCCCTGGGTCTTGATGGACAG |
PCR Primers
| Primer | Sequence |
| SU | AATTCCTAGATCAAATTAGC |
| SD | CTGTTGAGAAGGTCACAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 438 bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
