10722 Hspb7 Status: Cancelled
| Gene | Hspb7 |
| Chromosome | 4 |
| Deletion Start | 141,421,788 |
| Deletion End | 141,424,159 |
| Deletion Size | 2,372 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,422,309 | 141,422,330 | TGCGTAGACACAGATTGAGGTG |
| TUP | 141,422,333 | 141,422,355 | TGAGGCTCACCTCACTCCATCCC |
| TUR | 141,422,425 | 141,422,444 | TCAGGGTCTCTGCTCTTCTC |
| TDF | 141,423,909 | 141,423,930 | TTTGCTCACAAGTGCCAACTAC |
| TDP | 141,423,934 | 141,423,960 | AGGATGTGGACCCAACATCGGTGACCT |
| TDR | 141,423,966 | 141,423,986 | GAGGCTACCATCCTCTCGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTGCCCTCCTGTGTCGG |
| SD | CAGTCTACCTAGGCCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 382 bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 396 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
