10726 Tlr12 Status: ES cell colonies screened / QC no positives
| Gene | Tlr12 |
| Chromosome | 4 |
| Deletion Start | 128,618,452 |
| Deletion End | 128,615,716 |
| Deletion Size | 2,737 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,617,761 | 128,617,782 | TGGAGATTCTGGACCTGACAAG |
| TUP | 128,617,737 | 128,617,759 | ACACCACTGGATGCCGTGTGGCT |
| TUR | 128,617,684 | 128,617,703 | CCGTGGCAGTCTGTGCATAC |
| TDF | 128,615,840 | 128,615,858 | GCACCAGCTTCCGGGTTAC |
| TDP | 128,615,816 | 128,615,838 | ACAGACTGGCTCGGCTGCTTCGA |
| TDR | 128,615,794 | 128,615,814 | GCCACAGACAGTAGTCTCCTC |
PCR Primers
| Primer | Sequence |
| SU | CTTTCTCCTAACAGGAAGTG |
| SD | TGAGGATTTGAAGATCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
