10727 Ffar2 Status: Cancelled
| Gene | Ffar2 |
| Chromosome | 7 |
| Deletion Start | 30,820,110 |
| Deletion End | 30,820,010 |
| Deletion Size | 1,152 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,819,755 | 30,819,776 | TCCCGGTGCAGTACAAGTTATC |
| TUP | 30,819,734 | 30,819,754 | CCGCCGGCCACTGTATGGAGT |
| TUR | 30,819,705 | 30,819,725 | ACATGATCCAGGCCACCAGAG |
| TDF | 30,819,415 | 30,819,438 | GTGACGCTTCTTAATTTCCTGGTG |
| TDP | 30,819,389 | 30,819,414 | TGCTTTGGACCCTACAACATGTCCCA |
| TDR | 30,819,311 | 30,819,331 | AGGCTGGCATTGAGGGAACTG |
PCR Primers
| Primer | Sequence |
| SU | TGGGAAGTCAACTTGGTCTC |
| SD | TCTTTCCAGCATCACCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 410 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | 1394 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
