| Gene | Sectm1b |
| Chromosome | 11 |
| Deletion Start | 121,056,294 |
| Deletion End | 121,054,875 |
| Deletion Size | 1,420 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,055,808 | 121,055,826 | CGCCTCCAGGAAACTACTC |
| TUP | 121,055,773 | 121,055,800 | TCATGGCAGCTTCATATTCAAGGAGGCC |
| TUR | 121,055,737 | 121,055,756 | GTTTGCCCTGAGCATCTGTG |
| TDF | 121,055,294 | 121,055,317 | TCTCCAATTCCCTTCACAGTGTTC |
| TDP | 121,055,269 | 121,055,291 | TCTCAGGCCACATAGCTGGAGCC |
| TDR | 121,055,212 | 121,055,236 | CACGATTCCTGTAGAAGTGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTGGCTCATTGTTTCACC |
| SD | TATCCTCAGTTAAAAACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 426 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 359 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10728C-H12 | VGB6 | C57BL/6NTac | |
| 10728C-E3 | VGB6 | C57BL/6NTac | |
