10728 Sectm1b Status: ES cell colonies screened / QC positives
| Gene | Sectm1b |
| Chromosome | 11 |
| Deletion Start | 120,917,608 |
| Deletion End | 120,916,189 |
| Deletion Size | 1,420 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CGCCTCCAGGAAACTACTC |
| TUP | | | TCATGGCAGCTTCATATTCAAGGAGGCC |
| TUR | | | GTTTGCCCTGAGCATCTGTG |
| TDF | | | TCTCCAATTCCCTTCACAGTGTTC |
| TDP | | | TCTCAGGCCACATAGCTGGAGCC
|
| TDR | | | CACGATTCCTGTAGAAGTGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTGGCTCATTGTTTCACC |
| SD | TATCCTCAGTTAAAAACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 426 bp | - | + | + |
| NeoFwd | SD | 359 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
