10729 Ifna5 Status: Cancelled
| Gene | Ifna5 |
| Chromosome | 4 |
| Deletion Start | 88,835,509 |
| Deletion End | 88,842,421 |
| Deletion Size | 6,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,835,687 | 88,835,710 | GACAGAAAGGACTTTGGATTCCCA |
| TUP | 88,835,715 | 88,835,738 | AGAAGGTGGGTGCCCAGCAGATCC |
| TUR | 88,835,783 | 88,835,807 | CCTTTGATGTGAAGATGTTCAGGAC |
| TDF | 88,835,834 | 88,835,858 | CTCCTAGACTCATTCTGCAATGAAG |
| TDP | 88,842,200 | 88,842,226 | CATCAGCAGCTCAATGACCTCAAAGCC |
| TDR | 88,835,899 | 88,835,914 | CTGCACCCCGACCTGT |
PCR Primers
| Primer | Sequence |
| SU | CAAATTAGGAAGAAAAACCCTC |
| SD | AAGTGGATTCAGATACCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 6265 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
