| Gene | Rhox1 |
| Chromosome | X |
| Deletion Start | 37,222,240 |
| Deletion End | 37,213,993 |
| Deletion Size | 8,248 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,221,555 | 37,221,576 | CCATGCAGCCTACTGATGTTAC |
| TUP | 37,221,525 | 37,221,551 | TGGTTATCCCACCAAAGGTAGCAGGTA |
| TUR | 37,221,496 | 37,221,515 | CTGACGTGCTTGGTGTCTTC |
| TDF | 37,214,462 | 37,214,485 | TCCTTACAAGGACAATTTCAGTGC |
| TDP | 37,214,440 | 37,214,461 | TTGCAGGCAGGGCCTAGCACTC |
| TDR | 37,214,409 | 37,214,433 | CCACAGTCTCTGTTTAACCCATCAG |
PCR Primers
| Primer | Sequence |
| SU | TCTCCCACCTTGATCACTAC |
| SD | GACACATGAATAAACATGTCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10730B-D10 | VGB6 | C57BL/6NTac | |
