10735 Rspo3 Status: Cancelled
| Gene | Rspo3 |
| Chromosome | 10 |
| Deletion Start | 29,506,577 |
| Deletion End | 29,499,935 |
| Deletion Size | 6,663 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,505,760 | 29,505,779 | TTGTTCCTCACTGGCTCTTC |
| TUP | 29,505,733 | 29,505,759 | TGGAACTTCTGAAGCTGCTTGCTCTCC |
| TUR | 29,505,682 | 29,505,703 | TGGACCTTACACTACCTCACTG |
| TDF | 29,500,674 | 29,500,691 | CCGCAGCATGGGAAACAG |
| TDP | 29,500,646 | 29,500,673 | AAAGGGCACTGACTCTAGGACAAGGACC |
| TDR | 29,500,623 | 29,500,643 | TGGCAGGCAGAAAGGACTATC |
PCR Primers
| Primer | Sequence |
| SU | CTGCATGCTGATTCAGTAATTC |
| SD | ACTTCCCAAAAGTTCCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 504 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 432 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
