10737 Scube2 Status: Cancelled
| Gene | Scube2 |
| Chromosome | 7 |
| Deletion Start | 109,865,583 |
| Deletion End | 109,831,124 |
| Deletion Size | 34,460 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,864,869 | 109,864,888 | GGGCCTCTGGGACACTATTC |
| TUP | 109,864,844 | 109,864,867 | TACCTTGCACAGTCTGAGGGCTCA |
| TUR | 109,864,818 | 109,864,838 | TCCCTGGTCTCAAGCAAACAG |
| TDF | 109,831,779 | 109,831,798 | GTTCCACAGACACCAACGAG |
| TDP | 109,831,750 | 109,831,771 | TCAACAATGGAGGCTGCCAGCA |
| TDR | 109,831,700 | 109,831,721 | ACCCTGGGTGACACTGACATTC |
PCR Primers
| Primer | Sequence |
| SU | CGCAGGGCTCTGCTGACAG |
| SD | GCAGTCCTGATAGACAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
