10742 Map3k6 Status: ES cell colonies screened / QC no positives
Gene | Map3k6 |
Chromosome | 4 |
Deletion Start | 133,241,075 |
Deletion End | 133,252,742 |
Deletion Size | 11,668 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 133,241,527 | 133,241,551 | TTACGGTAAACTAAAGCCGAACTTG |
TUP | 133,241,555 | 133,241,583 | CAAGGCAGTAACGACAGGGCATTTAGTGG |
TUR | 133,241,645 | 133,241,664 | CCACGCTCACCGAAAGATAC |
TDF | 133,251,820 | 133,251,839 | TGCAGGCCCTTCCATTCTAC |
TDP | 133,251,843 | 133,251,865 | CCCTACCTTCCCACAGGCTTCGG |
TDR | 133,251,876 | 133,251,896 | GGCACTCACGTTCCTTCTCAG |
PCR Primers
Primer | Sequence |
SU | GACTTCCCAGGACTCTTCTG |
SD | CACTCAGACATGGAGACCTC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 365 bp | - | + | + |
TUF | TUR | 138 bp | + | + | - |
TDF | TDR | 77 bp | + | + | - |
NeoFwd | SD | 357 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |