10742 Map3k6 Status: ES cell colonies screened / QC no positives
| Gene | Map3k6 |
| Chromosome | 4 |
| Deletion Start | 133,241,075 |
| Deletion End | 133,252,742 |
| Deletion Size | 11,668 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,241,527 | 133,241,551 | TTACGGTAAACTAAAGCCGAACTTG |
| TUP | 133,241,555 | 133,241,583 | CAAGGCAGTAACGACAGGGCATTTAGTGG |
| TUR | 133,241,645 | 133,241,664 | CCACGCTCACCGAAAGATAC |
| TDF | 133,251,820 | 133,251,839 | TGCAGGCCCTTCCATTCTAC |
| TDP | 133,251,843 | 133,251,865 | CCCTACCTTCCCACAGGCTTCGG |
| TDR | 133,251,876 | 133,251,896 | GGCACTCACGTTCCTTCTCAG |
PCR Primers
| Primer | Sequence |
| SU | GACTTCCCAGGACTCTTCTG |
| SD | CACTCAGACATGGAGACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 365 bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
