10747 Olfr275 Status: Cancelled
| Gene | Olfr275 |
| Chromosome | 4 |
| Deletion Start | 52,825,378 |
| Deletion End | 52,826,390 |
| Deletion Size | 1,013 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,825,472 | 52,825,492 | TCACCGTATTTGTGCTGTGTC |
| TUP | 52,825,493 | 52,825,520 | TGCTGATGTATCTGATCACCTTGCTGGG |
| TUR | 52,825,556 | 52,825,576 | ACATGGGAGTGTGCAGATGAG |
| TDF | 52,826,087 | 52,826,107 | CAATGGATGGCCGAAGCAAAG |
| TDP | 52,826,111 | 52,826,135 | TTTCAACATGTGCAGCCCACTTGAC |
| TDR | 52,826,137 | 52,826,159 | GTCCCATAGAACAGAACCACCAC |
PCR Primers
| Primer | Sequence |
| SU | GAATTCTTCTCTCGCTCCATC |
| SD | GATGGTCATATGTACATGCTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 469 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
