10749 Taar6 Status: ES cell clone microinjected
| Gene | Taar6 |
| Chromosome | 10 |
| Deletion Start | 23,705,449 |
| Deletion End | 23,704,415 |
| Deletion Size | 1,035 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,705,064 | 23,705,085 | TGAGCTTCATCTCCATCGACAG |
| TUP | 23,705,039 | 23,705,061 | ACATCGCTGTCACGGACCCTCTG |
| TUR | 23,705,017 | 23,705,038 | CCGTGAACTTGGTGGGATAGAC |
| TDF | 23,704,544 | 23,704,567 | TGTGTTTGGTGTGCTTATTACAAC |
| TDP | 23,704,518 | 23,704,543 | TCAGCCATGAACCCTCTGATCTACGC |
| TDR | 23,704,436 | 23,704,457 | GTTCATAGTGGCTGAGCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CCATTATTTTGGACCATAGGAGC |
| SD | GTTCAGCTCTGAGAAGAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10749A-D5 | VGB6 | C57BL/6NTac | |
| 10749A-D6 | VGB6 | C57BL/6NTac | |
| 10749A-E2 | VGB6 | C57BL/6NTac | |
| 10749A-G3 | VGB6 | C57BL/6NTac | |
| 10749A-H10 | VGB6 | C57BL/6NTac | |
| 10749A-H11 | VGB6 | C57BL/6NTac | |
