10754 Insl6 Status: ES cell colonies screened / QC no positives
| Gene | Insl6 |
| Chromosome | 19 |
| Deletion Start | 29,325,312 |
| Deletion End | 29,321,397 |
| Deletion Size | 3,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,325,286 | 29,325,307 | GCTGTGCTGTTCTTGTCTGTTG |
| TUP | 29,325,259 | 29,325,285 | TGGCTTGGACTCCTACTGACTCCTTTC |
| TUR | 29,325,226 | 29,325,246 | CCTGGGTCTGGATTCCTCTTC |
| TDF | 29,321,674 | 29,321,694 | CTCAGTCACTGCCCGACTATC |
| TDP | 29,321,642 | 29,321,669 | TGAAAAGACGGAGTTGCTTCCTAAGGCA |
| TDR | 29,321,618 | 29,321,638 | GCCACTGTGGTATGAAAACAC |
PCR Primers
| Primer | Sequence |
| SU | GCATTTCTGAGGCCAAAAGAG |
| SD | ACCTACACGGATTATGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 460 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
