10757 Arhgef15 Status: ES cell colonies screened / QC positives
| Gene | Arhgef15 |
| Chromosome | 11 |
| Deletion Start | 68,768,523 |
| Deletion End | 68,758,067 |
| Deletion Size | 10,457 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,767,667 | 68,767,688 | GCTTAGGCAATCCCTTCTTCAC |
| TUP | 68,767,645 | 68,767,666 | AGATGCTGCCCTGGCCTGCCCT |
| TUR | 68,767,614 | 68,767,633 | TTGCTACATGGCAGACACAG |
| TDF | 68,758,926 | 68,758,946 | TGCTGTGCAATAGGAACTGTC |
| TDP | 68,758,901 | 68,758,922 | AGGCCAGTGTGCCCAGACTGAC |
| TDR | 68,758,878 | 68,758,899 | GCTTCCATCATTCCCTTCTCTG |
PCR Primers
| Primer | Sequence |
| SU | GGAAAAGCTGCCCAGGAAG |
| SD | ATGCCCCTGCTTAATCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 402 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10757A-D2 | VGB6 | C57BL/6NTac | |
| 10757B-C6 | VGB6 | C57BL/6NTac | |
| 10757B-G3 | VGB6 | C57BL/6NTac | |
