10758 C1qc Status: Cancelled
| Gene | C1qc |
| Chromosome | 4 |
| Deletion Start | 136,892,559 |
| Deletion End | 136,889,969 |
| Deletion Size | 2,591 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,891,785 | 136,891,806 | GTTGTGGCCCTTTATGAGTGTG |
| TUP | 136,891,759 | 136,891,782 | TGCTTTCCCTGGGAGATCAGTGGA |
| TUR | 136,891,722 | 136,891,741 | TCTGCTCAGCCCTACTTACC |
| TDF | 136,890,271 | 136,890,292 | GGGCCTCTACTACTTCGTCTAC |
| TDP | 136,890,246 | 136,890,269 | ACACATCGCATACGGCCAACCTGT |
| TDR | 136,890,220 | 136,890,240 | GGCAAGGTTGAGGTTCAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TCCTGAACCATAGGACTAGG |
| SD | GAGGTCTTCAAGTGAGAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
