10759 Tslp Status: ES cell colonies screened / QC positives
| Gene | Tslp |
| Chromosome | 18 |
| Deletion Start | 32,975,057 |
| Deletion End | 32,978,820 |
| Deletion Size | 3,764 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCTGACAACAGATATGGATATTGG |
| TUP | | | TGCTTGGTACAGAATGGGAATCCTTGTGA |
| TUR | | | CTTAGCTGTCCCTCTCCTGAG |
| TDF | | | CCCTAACAGATGTGCAGATAGACAC |
| TDP | | | AAACAGCCAGGGCTCAGGAGACAAACA |
| TDR | | | TTTGGTCTGGGAAGGAGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAAGTGTTTAGGGCAGAGC |
| SD | CACCTCATCATGGCAGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
