| Gene | Tslp |
| Chromosome | 18 |
| Deletion Start | 32,815,403 |
| Deletion End | 32,819,166 |
| Deletion Size | 3,764 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,815,942 | 32,815,966 | GGCTGACAACAGATATGGATATTGG |
| TUP | 32,815,972 | 32,816,000 | TGCTTGGTACAGAATGGGAATCCTTGTGA |
| TUR | 32,816,009 | 32,816,029 | CTTAGCTGTCCCTCTCCTGAG |
| TDF | 32,818,242 | 32,818,266 | CCCTAACAGATGTGCAGATAGACAC |
| TDP | 32,818,274 | 32,818,300 | AAACAGCCAGGGCTCAGGAGACAAACA |
| TDR | 32,818,353 | 32,818,374 | TTTGGTCTGGGAAGGAGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAAGTGTTTAGGGCAGAGC |
| SD | CACCTCATCATGGCAGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10759A-B7 | VGB6 | C57BL/6NTac | |
| 10759A-E4 | VGB6 | C57BL/6NTac | |
| 10759A-H7 | VGB6 | C57BL/6NTac | |
