10760 Metrn Status: Cancelled
| Gene | Metrn |
| Chromosome | 17 |
| Deletion Start | 25,796,959 |
| Deletion End | 25,794,952 |
| Deletion Size | 2,008 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,796,327 | 25,796,345 | CACCACACCGCGACATCAG |
| TUP | 25,796,305 | 25,796,326 | CCGCAGAGTTGCTGCCTTCCGT |
| TUR | 25,796,281 | 25,796,301 | ACGTTGGTCCTCGTGCAGTTC |
| TDF | 25,795,345 | 25,795,364 | TCTGTTGCGGGAGAGCTTAG |
| TDP | 25,795,320 | 25,795,342 | TCTGCCTCACATTCCCACGTGCC |
| TDR | 25,795,282 | 25,795,303 | CCGTGGATCACTACGAGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | GGGAAACAGGAGCGCTTTC |
| SD | CAGGCTAAGAAGGGTTAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 423 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
