| Gene | Akr1b10 |
| Chromosome | 6 |
| Deletion Start | 34,384,345 |
| Deletion End | 34,396,654 |
| Deletion Size | 12,329 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,384,969 | 34,384,989 | CACCCTGGCTGTGTTCAACTC |
| TUP | 34,384,991 | 34,385,014 | CCTGGCAAGTTTCTCCGAGGCTCA |
| TUR | 34,385,041 | 34,385,064 | GGATGTGTAATTTCTGGCTTGGTC |
| TDF | 34,396,495 | 34,396,518 | TTATGGGAGAAGAGGCTGAGAAAG |
| TDP | 34,396,525 | 34,396,552 | CACGCTGAATAGACCACCTAAGAGTGCA |
| TDR | 34,396,561 | 34,396,581 | TTCACGGAAGCAATGGGTATG |
PCR Primers
| Primer | Sequence |
| SU | TCTGCAGCAGGGAAACTTAG |
| SD | GGCCAGGTCAAATTAGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 299 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10761A-B1 | VGB6 | C57BL/6NTac | |
| 10761A-E7 | VGB6 | C57BL/6NTac | |
| 10761A-E9 | VGB6 | C57BL/6NTac | |
| 10761A-F2 | VGB6 | C57BL/6NTac | |
| 10761A-F10 | VGB6 | C57BL/6NTac | |
| 10761A-H4 | VGB6 | C57BL/6NTac | |
