10765 Zfp69 Status: Cancelled
| Gene | Zfp69 |
| Chromosome | 4 |
| Deletion Start | 120,935,097 |
| Deletion End | 120,930,328 |
| Deletion Size | 4,770 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,934,301 | 120,934,323 | GGGTAGATGAGCCCTTTGGATAG |
| TUP | 120,934,272 | 120,934,294 | CAGAGTGCTCGGTCTTCCCAGGC |
| TUR | 120,934,251 | 120,934,270 | GTGCCCTAAGCCTTCCAGAC |
| TDF | 120,930,541 | 120,930,561 | CCTACGAGTGTAACCGCTGTG |
| TDP | 120,930,515 | 120,930,538 | AAAGCCTACAGGCACGACTCCTCC |
| TDR | 120,930,490 | 120,930,509 | TGTGATGTCGCTGGTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | CACCTCAACTCTCACTGGTG |
| SD | CAGTAGTTCCCAAATACTAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 440 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
