| Gene | Rassf8 |
| Chromosome | 6 |
| Deletion Start | 145,808,463 |
| Deletion End | 145,817,202 |
| Deletion Size | 8,758 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,808,475 | 145,808,492 | TGGGTGGATGGCGTTCAG |
| TUP | 145,808,496 | 145,808,520 | ATCGTCTGTGGCGTCACGGAAGTCA |
| TUR | 145,808,535 | 145,808,558 | AGCTTGGGCTAAGGCTATTACAAC |
| TDF | 145,816,504 | 145,816,524 | GAGCAGGAGCTAGAGCAGTTG |
| TDP | 145,816,525 | 145,816,546 | ACCAAAGAGCTGCGGCAGGTCA |
| TDR | 145,816,557 | 145,816,575 | CCCTGTCTGCTGGATGAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGTGTACAATGCTAACCAGG |
| SD | CAGCTGCTGGACAACATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 263 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10768A-D12 | VGB6 | C57BL/6NTac | |
| 10768A-E12 | VGB6 | C57BL/6NTac | |
