10771 Mcoln3 Status: Cancelled
| Gene | Mcoln3 |
| Chromosome | 3 |
| Deletion Start | 146,121,777 |
| Deletion End | 146,140,598 |
| Deletion Size | 18,822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 146,121,797 | 146,121,818 | CAAATCCCGAGGTGCTGGTTAG |
| TUP | 146,121,819 | 146,121,842 | CAGCTGCAGAGCTCGCCAAGATGA |
| TUR | 146,121,903 | 146,121,924 | GAACTTGAGTTTCCGCCTCATC |
| TDF | 146,140,455 | 146,140,476 | TCTGTGTAGCACTACCAGCAAG |
| TDP | 146,140,477 | 146,140,502 | ATGGCTTCCCAGAGACGGAACTTCGA |
| TDR | 146,140,554 | 146,140,574 | AGAACCCGGAGGGTCATCTTC |
PCR Primers
| Primer | Sequence |
| SU | TCTGCTGGTGAGAGGCTTG |
| SD | AGGCACAAGGCTTGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 359 bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | 360 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
