| Gene | Tmem91 |
| Chromosome | 7 |
| Deletion Start | 25,673,631 |
| Deletion End | 25,669,323 |
| Deletion Size | 4,327 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,673,595 | 25,673,614 | GGAACTCCAGCAACCCTTAC |
| TUP | 25,673,571 | 25,673,594 | TGCCAAGCATAACCTGTGACCTCC |
| TUR | 25,673,544 | 25,673,563 | GCTCTGGCTTCTCAGACCTG |
| TDF | 25,670,196 | 25,670,215 | CGAGGATGCAGCTCAGTTGG |
| TDP | 25,670,173 | 25,670,195 | CAGAGCACTCAACCCGGCACTCA |
| TDR | 25,670,125 | 25,670,145 | CACCCTGCCTTCGTCTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTAATCCCAGAATTGATCACC |
| SD | CCAGCTAACCTAGCTCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10774A-B6 | VGB6 | C57BL/6NTac | |
| 10774A-D7 | VGB6 | C57BL/6NTac | |
| 10774A-F2 | VGB6 | C57BL/6NTac | |
| 10774A-H4 | VGB6 | C57BL/6NTac | |
