10775 Ap1g1 Status: Cancelled
| Gene | Ap1g1 |
| Chromosome | 8 |
| Deletion Start | 109,802,953 |
| Deletion End | 109,819,037 |
| Deletion Size | 16,085 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,803,276 | 109,803,296 | TGTGACAGCAAGGGTAGGATG |
| TUP | 109,803,298 | 109,803,321 | AGGGAGCAACCGTGAACCTGGAAA |
| TUR | 109,803,333 | 109,803,352 | ACAGTGGGAAGGCAGATGAG |
| TDF | 109,818,090 | 109,818,112 | TCCATTTCCCTTGAGCCCATTAG |
| TDP | 109,818,114 | 109,818,141 | TGAACTCATAGTGGACAGCAGTCTCCCA |
| TDR | 109,818,143 | 109,818,161 | CAGGAAGCACCAGCCAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGATGTTCTATGTCCTGTCTTG |
| SD | AAATCCAGATTTCACCAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 382 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
