10777 Ap3d1 Status: Germline transmission achieved
| Gene | Ap3d1 |
| Chromosome | 10 |
| Deletion Start | 80,204,733 |
| Deletion End | 80,170,615 |
| Deletion Size | 34,119 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,203,848 | 80,203,867 | GGAAGGCCATGCTGAAAGAG |
| TUP | 80,203,813 | 80,203,839 | TCCCTAGCTGGGACAGTCATTTCTCTG |
| TUR | 80,203,784 | 80,203,807 | ACCAGGTAGGGTAGAAGGGAAGAG |
| TDF | 80,171,268 | 80,171,287 | TGCAGTTGTGGAGCGAGTAG |
| TDP | 80,171,241 | 80,171,262 | TGTGCCTCCATGTACAGCCGCT
|
| TDR | 80,171,221 | 80,171,240 | GACATGGTGGCCCTGGATAG |
PCR Primers
| Primer | Sequence |
| SU | AGGCTCTCCGTCAGCTGAC |
| SD | TCTGGCTAGGGTCAGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 399 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 48 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10777A-C7 | VGB6 | C57BL/6NTac | |
| 10777A-D10 | VGB6 | C57BL/6NTac | |
