10781   4933428G20Rik     Status: ES cell colonies screened / QC positives

Gene4933428G20Rik
Chromosome11
Deletion Start97,492,771
Deletion End97,491,006
Deletion Size1,766
Genome Build38
MGI IDMGI:1918475
NCBI Gene ID0
BAC ID332F8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,492,16697,492,189CCCAGCCAGTATCTCAGAACTAAG
TUP97,492,13897,492,163TGCTGTGAGGTCTTTCTCTGGGACAA
TUR97,492,08697,492,106GCCACAGCTCATCTCACATTC
TDF97,491,16997,491,189GGCAGGAGGTACTCGATGTTG
TDP97,491,14297,491,166CACCGACTGTGGCTCCTTGTCATCA
TDR97,491,11197,491,130CTGACGCCTCCCTCTGCTTG

PCR Primers

PrimerSequence
SUCAGGTGGTGGAATCTGCTG
SDCCAAGGGAAGATTCTGGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev406 bp-++
TUFTUR104 bp++-
TDFTDR79 bp++-
NeoFwdSD300 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10781A-A8VGB6C57BL/6NTac
10781A-B10VGB6C57BL/6NTac
10781A-B11VGB6C57BL/6NTac
10781A-D2VGB6C57BL/6NTac
10781A-D3VGB6C57BL/6NTac
10781A-F9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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