| Gene | Gm13088 |
| Chromosome | 4 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,656,119 | 143,656,141 | GCTACACCTGCATCCCTTAAAGT |
| TUP | 143,656,079 | 143,656,107 | TGACATTGGGTGTAAAGGGCTTGGAATGA |
| TUR | 143,656,005 | 143,656,029 | CCTCTAGCCTCATGCTCAGTAAATG |
| TDF | 143,654,293 | 143,654,314 | CTTCTACAGCACACATCCAAGT |
| TDP | 143,654,265 | 143,654,292 | TGACACAGCTGAGCTATGAGATGTACCC |
| TDR | 143,654,173 | 143,654,191 | TGGCCCTGAGCATATCCAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGAGGGTGCTCAAAATGG |
| SD | GTGTTTCTATTGTAACACACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 142 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
