10785   Pramef17     Status: ES cell colonies screened / QC no positives

GenePramef17
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:3650199
NCBI Gene ID626943
BAC ID74G24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCACCTTTAATCCCAGCACTTGGT
TUPCAGGTGAATATCTGAATTTGAGGCCAGCC
TURCTGTGTATCCCTGCCTCTGTAG
TDFCAACCATGTGATCTCTCCCTAGGTA
TDPTGGAGGCTCCCTTGTTGTCCCTG
TDRGTTCCAATGCTGGGAAAATGAC

PCR Primers

PrimerSequence
SUTGAAGCAAAGGAAAGGGAGAG
SDACAGTATCCCTCCATCTCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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