10786   Gm13101     Status: Cancelled

GeneGm13101
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:3650204
NCBI Gene ID626922
BAC ID74G24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF143,930,422143,930,450GGTAAACAAGAACTATGTAATGCTAAACA
TUP143,966,556143,966,580CGCGTAAGGAGAAGGACATCTGGGC
TUR143,966,474143,966,495CAACAACTGTCCAGGACAGTGT
TDF143,949,474143,949,496TCACAGCCTAGAAATGGGACTTG
TDP143,949,446143,949,471TTGGCTGACTCAAACAGGTTGCACTG
TDR143,949,416143,949,437CAGTGTCACCACAGGATGGTAG

PCR Primers

PrimerSequence
SUTGGCTCATCACTAAATGACTG
SDAAGTAGAAAGAAAGACTGGAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR36074 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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