10789 Gm13128 Status: ES cell colonies screened / QC no positives
| Gene | Gm13128 |
| Chromosome | 4 |
| Deletion Start | 144,330,232 |
| Deletion End | 144,333,189 |
| Deletion Size | 2,958 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,330,483 | 144,330,504 | CAGGCTATACTGGAGGGAGTTG |
| TUP | 144,330,510 | 144,330,534 | TGGCTGAAACGGAAGTATCGTCCCA |
| TUR | 144,330,536 | 144,330,558 | TCCCTACCTGGTATTTGGCTTAC |
| TDF | 144,332,415 | 144,332,434 | CCCTGCTTGTCACTGAAGAC |
| TDP | 144,332,444 | 144,332,466 | TGGCTGTGGAATGAGCCAGTCCC |
| TDR | 144,332,523 | 144,332,543 | TGGGATGGCATCCAGTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | GATTCAGAGTAGCAAAGTTTGG |
| SD | CGCTTAGACAACACAGTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 129 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
