10791 Gm14443 Status: Cancelled
| Gene | Gm14443 |
| Chromosome | 2 |
| Deletion Start | 175,171,971 |
| Deletion End | 175,168,555 |
| Deletion Size | 3,417 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 175,132,483 | 175,132,512 | GAGATGGACAGTTTATAAGATTCAAGAATC |
| TUP | 175,171,124 | 175,171,143 | TGTGGTAAAACCTTAACTCT |
| TUR | 175,132,408 | 175,132,433 | TCCATTTTATATTCACACAGCTGACT |
| TDF | 175,169,257 | 175,169,279 | AGCGAACACATACAGGAGAGCAG |
| TDP | 175,169,236 | 175,169,256 | CCTTATGAATGTAACCAATGT |
| TDR | 175,169,201 | 175,169,219 | ATTTGGAGGTCCCCACTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGAAACTGCAGTCCTAAGC |
| SD | GGGTTTCTCTCAAGTGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 2334780 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 2866371 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
