10795 Gm12790 Status: Cancelled
| Gene | Gm12790 |
| Chromosome | 4 |
| Deletion Start | 101,968,212 |
| Deletion End | 101,967,437 |
| Deletion Size | 776 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,967,956 | 101,967,976 | TGTGTTGGATGCAGTGGATTG |
| TUP | 101,967,929 | 101,967,953 | TGAGTAGCCAGAAGGTTCGGCCTAA |
| TUR | 101,967,894 | 101,967,914 | CAGCTTTGCCTTCCATACGAC |
| TDF | 101,967,774 | 101,967,794 | TTTGACCCACAGGAGGTGTAG |
| TDP | 101,967,733 | 101,967,762 | TCAATTTGGTGGATGTCAACTGTGACTTCC |
| TDR | 101,967,698 | 101,967,717 | TCGGAGAAGATCCCGTGTTG |
PCR Primers
| Primer | Sequence |
| SU | CTGTTTGCTCCGGTTAACCC |
| SD | TGGCAGACATACAAAACCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 432 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
