10799 Baat Status: Cancelled
| Gene | Baat |
| Chromosome | 4 |
| Deletion Start | 49,503,117 |
| Deletion End | 49,489,748 |
| Deletion Size | 13,370 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,502,752 | 49,502,773 | CATAGTCGTCAGTCCTCCCTTG |
| TUP | 49,502,718 | 49,502,746 | CCTGACTCTGGAAAGGTGGTATGTGGCAC |
| TUR | 49,502,678 | 49,502,697 | CGGATGCGGCTTTCCTTTAC |
| TDF | 49,490,236 | 49,490,258 | CCCACCTGTACCCAGTAATGAAG |
| TDP | 49,490,204 | 49,490,231 | TGTAGTCACCAATGCCTTGGGACTTGTA |
| TDR | 49,490,175 | 49,490,198 | GCAGTTTCCTGAAAGGTTCGATAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCCCTCTGGCTTCTAG |
| SD | AACAGAAAGTTGCTATGATGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
